1. Gabatarwa
Jimlar kayan haɓaka ko da sauri na acial ta dace da cire jimlar ƙarfin lantarki ko da alama daga magani, plasma, nama homogenate da sauran samfurori. Kit ɗin ya dogara ne akan fasahar tsarkakewar silica, wanda ke kawar da buƙatar hakar mai guba-chloriform da cigaban giya lokaci-lokaci. Wannan samfurin ya samu nasarar fitar da nasarar makiyaya daga hepatitis ba / c, hepatitis c, da kuma misali na Norvirus. Za'a iya amfani da DNA / RNA a cikin jerin abubuwan gwaje-gwaje na ƙasa kamar PCR, RT-PCR, da fitila.
Lura:
1. An narkar da mai ɗaukar ruwa mai ƙarfi a cikin ruwa kyauta kyauta zuwa 1μg / μl kafin amfani da, da kuma vortex to narke. Adana cikin AT -70 ° C. Idan kana buƙatar adana shi a -20 ℃ na dogon lokaci, don Allah a kunna shi bisa ga yawan lokutan amfani.
2. Narke kayan adon zamani k Ana iya adana foda mai narkewa a 2-8 ° C na shekara guda, amma narkar da furotin K dole ne a adana shi cikin AC20 ° C. Maimaita daskarewa da narkewar furotin K na iya shafar ayyukan sa.
3. Dole ne a dilika vhb vhb da 14 ml cikakken ethanol 14 kafin amfani da adana shi a zazzabi a ɗakin.
4. Buffer RW2 dole ne a diluted tare da 80 ml na cikakken ethanol kafin amfani da adana shi a zazzabi a ɗakin.
3. Shirancin Shirdan
Ban da furotin aeseinase k da mai ɗaukar nauyin wannan samfurin za'a iya adanar shi a zazzabi a ɗakin (15-25 ° C) na tsawon watanni 12, kuma ya kamata a adana su a 2-8 ° C don ajiya na dogon lokaci. Proteinase K da Carrier RNA bushe foda ana jigilar su a zazzabi a daki. Da fatan za a adana A -20 ° C bayan karbar samfurin gwajin, kuma adana AT -20 ° C bayan narkewa.
